Integrated metabolite and gene expression profiles identify lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes.

Integrated metabolite and gene expression profiles identify lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes.

OBJECTIVE
We combined gene expression and metabolic profile analysis to identify factors associated with the outcomes of patients with hepatocellular carcinoma (HCC).


METHOD
We compared the pattern of metabolism and gene expression between paired tumor and nontumor tissue from 30 patients with HCC, and validated the results using a sample of 356 patients with HCC. A total of 469 metabolites were measured by using chromatography / mass spectrometry spectrometry liquid and gas chromatography / mass. Metabolism and integrated genomic data, and Kaplan-Meier and Cox proportional hazards analysis was used to a certain pattern associate with patient outcomes. related factors were evaluated for their effect on cancer cells in vitro and tumor formation in nude mice.


RESULTS
We identified 28 metabolites and 169 genes associated with aggressive HCC. lipid metabolite-stearoyl-CoA desaturase (SCD) is associated with the aberrant activity of palmitate signal in aggressive HCC samples. The expression of gene products associated with these metabolites, including SCD, was independently associated with survival time and recurrence of tumors in test and validation sets.

The combined expression of SCD and α-fetoprotein associated with outcomes of patients with early-stage liver cancer. Levels of monounsaturated palmitic acid, the product of SCD activity, an increase in aggressive HCCS; monounsaturated palmitic acid increased migration and invasion of HCC cells cultured and colony formation by liver cancer cells. HCC cells expressed small interfering RNA against SCD decreased cell migration and the formation of colonies in culture and reduced tumorigenicity in mice.


CONCLUSION
By using a combination of gene expression and metabolic profile analysis, we identified a network of lipogenic involving SCD and palmitic signal and associated with the development of HCC and patient outcomes. Microarray platform and data that has been submitted to the Gene Expression Omnibus at NCBI public database following the guidelines MIAME. accession numbers: GPL4700 (platform), and GSE6857 (sample).

Integrated metabolite and gene expression profiles identify lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes.
Integrated metabolite and gene expression profiles identify lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes.

Interferome v2.0: updated database of interferon-regulated genes described.

Interferome v2.0 is an update of an earlier version of DB Interferome NAR published in the 2009 edition of the database. Much better computing infrastructure now allows more complex and faster queries, and supports more data sets of types I, II and III interferon (IFN) -treated cells, rat or human.

Quantitative, MIAME appropriate data is collected, carried out a thorough analysis, standardized, quantitative and statistical and then significant changes in gene expression are uploaded. a comprehensive manual collection of metadata in v2.0 allows flexible, detailed searches capacity including the parameters: range double change, the type of IFN, concentration and time, and cell / tissue types.

WSE-5400-UP Printgraph Classic

2305403 1unit
EUR 10149.00
Description: M ai n uni t , Cont r ol , U V, Ther mal Pr i nt er

WSE-5400-CyP Printgraph Classic

2305407 1unit
EUR 10697.00
Description: M ai n uni t , Cont r ol , CyanL ED, Ther mal Pr i nt er

Classic CRISPR sgRNA Synthesis Kit

K1252-25 25 Reactions
EUR 439.00
  • Kit components:
  • 2X Classic sgRNA Synthesis SuperMix
  • Scaffold Template and Primer
  • sgRNA Control Oligo (10 ?M)
  • OneScribe Enzyme Mix
  • DNase I, RNase-Free (2 U/?l)
  • 10X DNase I Reaction Buffer
  • DistantTM PCR Mix (#M1136-200)
  • Nuclease-free H2O
Description: Classic CRISPR sgRNA Synthesis Kit

Human Drug Detoxication I

HDTX-I 1 set
EUR 548.00

Human Kinase Library I

HKIN-I 1 set
EUR 450.00

Human Cytokine Primer Library I

HCA-I 1 set
EUR 450.00

Mouse Cytokine Primer Library I

MCA-I 1 set
EUR 450.00

Rat Cytokine Primer Library I

RCA-I 1 set
EUR 548.00

Human Colon Cancer Primer Library I

HCCP-I 1 set
EUR 548.00

Human DNA Repair Primer Library I

HDRL-I 1 set
EUR 548.00

Human Drug Transporter I Primer Library

HDTP-I 1 set
EUR 548.00

Mouse DNA Repair Primer Library I

MDRL-I 1 set
EUR 645.00

Human Cancer Driver Gene I Primer Library

HCDG-I 1 set
EUR 645.00

Human Interferon Type I Signaling Primer Library

HIFN-I 1 set
EUR 548.00

Steady-Luc Firefly HTS Assay Kit (Lyophilized)

30028-L1 120TST
EUR 139.00
Description: Minimum order quantity: 1 unit of 120TST

Steady-Luc Firefly HTS Assay Kit (Lyophilized)

30028-L2 1000TST
EUR 610.00
Description: Minimum order quantity: 1 unit of 1000TST

Steady-Luc Firefly HTS Assay Kit (Lyophilized)

30028-L3 10000TST
EUR 3145.00
Description: Minimum order quantity: 1 unit of 10000TST

Nitric Oxide Cell-Based HTS Assay Kit

K979-100
EUR 653.00

AXYPREP FRAGMENTSELECT-I KIT- 1 ML - (SAMPLE SIZE)

MAG-FRAG-I-1 1/pk
EUR 64.00
Description: Bioscience Mag Beads; Magnetic Fragment Select

AXYPREP FRAGMENTSELECT-I KIT- 250 ML - 1900 PREPS

MAG-FRAG-I-250 1/pk
EUR 1953.00
Description: Bioscience Mag Beads; Magnetic Fragment Select

AXYPREP FRAGMENTSELECT-I KIT- 5 ML - 4 PREPS

MAG-FRAG-I-5 1/pk
EUR 158.00
Description: Bioscience Mag Beads; Magnetic Fragment Select

AXYPREP FRAGMENTSELECT-I KIT- 50 ML - 380 PREPS

MAG-FRAG-I-50 1/pk
EUR 659.00
Description: Bioscience Mag Beads; Magnetic Fragment Select

TW,HTS,6.5MM,0.4UM,S,IND,1/2

3396 1/pk
EUR 399.00
Description: Permeable Support; HTS Transwell

TW,HTS,6.5MM,0.4UM,S,BK,12/12

3397 12/pk
EUR 1955.00
Description: Permeable Support; HTS Transwell

TW,HTS,6.5MM,3.0UM,S,IND,1/2

3398 1/pk
EUR 399.00
Description: Permeable Support; HTS Transwell

TW,HTS,6.55MM,3.0UM,S,BK,12/12

3399 12/pk
EUR 1955.00
Description: Permeable Support; HTS Transwell

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

30009-1 1KIT
EUR 100.00
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

30009-2 1KIT
EUR 226.00
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

30009-3 1KIT
EUR 809.00
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

30012-1 1KIT
EUR 100.00
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

30012-2 1KIT
EUR 358.00
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

30012-3 1KIT
EUR 1627.00
Description: Minimum order quantity: 1 unit of 1KIT

Steady-Luc Firefly HTS Assay Kit (12 ml)

30028-1 1KIT
EUR 139.00
Description: Minimum order quantity: 1 unit of 1KIT

Steady-Luc Firefly HTS Assay Kit (100 ml)

30028-2 1KIT
EUR 610.00
Description: Minimum order quantity: 1 unit of 1KIT

Steady-Luc Firefly HTS Assay Kit (10x100 ml)

30028-3 1KIT
EUR 3145.00
Description: Minimum order quantity: 1 unit of 1KIT

TW,HTS,CULTURE RESERVOIR,NT,S,BK,12PL/48

3395 12/pk
EUR 295.00
Description: Permeable Support; HTS Transwell

3D Cell Culture HTS Cell Viability Complete Assay Kit

K948-100
EUR 343.00

TRANSWELL,96WL,HTS,W/3.0 MEMBRANE,S,IND,1/2

3385 1/pk
EUR 179.00
Description: Permeable Support; HTS Transwell

TRANSWELL,96WL,HTS,W/3.0 MEMBRANE,S,BK,4/8

3386 4/pk
EUR 626.00
Description: Permeable Support; HTS Transwell

TRANSWELL,96WL, HTS,W/5.0UM MEMBRANE,S,BK,4/8

3387 4/pk
EUR 626.00
Description: Permeable Support; HTS Transwell

TRANSWELL,96WL,HTS,W/5.0 MEMBRANE,S,BK,1/2

3388 1/pk
EUR 179.00
Description: Permeable Support; HTS Transwell

Steady-Luc Firefly HTS Assay Kit (trial size)(4 mL)

30028-T 1KIT
EUR 94.00
Description: Minimum order quantity: 1 unit of 1KIT

HTS TRANSWELL,TISSUE CULTURE TREATED RESERVOIR,S,BK,12/48

4395 12/pk
EUR 321.00
Description: Permeable Support; HTS Transwell

TRANSWELL,96WL,WHITE,HTS RECEIVER,TCT,S,BK,10/10

3783 10/pk
EUR 158.00
Description: Permeable Support; HTS Transwell

Human Breast Slide (Normal) (5 slides/pk)

HTS-10001 1 pk
EUR 286.00

Human Breast Slide (Benign) (5 slides/pk)

HTS-10003 1 pk
EUR 286.00

Human Breast Slide (Fibroadenoma) (5 slides/pk)

HTS-10004 1 pk
EUR 286.00

Human Breast Slide (Carcinoma) (5 slides/pk)

HTS-10009 1 pk
EUR 286.00

Human Breast Slide (Ductal Carcinoma In Situ (DCIS)) (5 slides/pk)

HTS-10010 1 pk
EUR 286.00

Human Breast Slide (Well differentiated Carcinoma) (5 slides/pk)

HTS-10012 1 pk
EUR 286.00

Human Breast Slide (Poorly differentiated carcinoma) (5 slides/pk)

HTS-10013 1 pk
EUR 286.00

Human Breast Slide (Abnormal) (5 slides/pk)

HTS-10020 1 pk
EUR 286.00

Human Lung Slide (Normal) (5 slides/pk)

HTS-10101 1 pk
EUR 286.00

Human Lung Slide (Tumor) (5 slides/pk)

HTS-10102 1 pk
EUR 286.00

Human Lung Slide (Small cell carcinoma) (5 slides/pk)

HTS-10103 1 pk
EUR 286.00

Human Lung Slide (Non-small cell carcinoma) (5 slides/pk)

HTS-10104 1 pk
EUR 286.00

Human Lung Slide (Squamous cell carcinoma) (5 slides/pk)

HTS-10105 1 pk
EUR 286.00

Human Lung Slide (Adenocarcinoma) (5 slides/pk)

HTS-10106 1 pk
EUR 286.00

Human Lung Slide (Abnormal) (5 slides/pk)

HTS-10110 1 pk
EUR 286.00

Human Liver Slide (Normal) (5 slides/pk)

HTS-10201 1 pk
EUR 286.00

Human Liver Slide (Malignant Tumor) (5 slides/pk)

HTS-10220 1 pk
EUR 286.00

Human Liver Slide (Hepatocellular Carcinoma) (5 slides/pk)

HTS-10222 1 pk
EUR 286.00

Human Liver Slide (Abnormal) (5 slides/pk)

HTS-10237 1 pk
EUR 286.00

Human Liver Slide (Necrotic tissue) (5 slides/pk)

HTS-10246 1 pk
EUR 286.00

Human Brain Slide (Normal) (5 slides/pk)

HTS-10301 1 pk
EUR 286.00

Human Brain Slide (Tumor) (5 slides/pk)

HTS-10302 1 pk
EUR 286.00

Human Kidney Slide (Normal) (5 slides/pk)

HTS-10401 1 pk
EUR 286.00

Human Kidney Slide (Tumor) (5 slides/pk)

HTS-10402 1 pk
EUR 286.00

Human Kidney Slide (Renal Cell Neoplasia) (5 slides/pk)

HTS-10403 1 pk
EUR 286.00

Human Kidney Slide (Clear Cell Renal Cell Carcinoma) (5 slides/pk)

HTS-10405 1 pk
EUR 286.00

Human Kidney Slide (Papillary Renal Cell Carcinoma) (5 slides/pk)

HTS-10407 1 pk
EUR 286.00

Human Kidney Slide (Renal Cell Carcinoma) (5 slides/pk)

HTS-10414 1 pk
EUR 286.00

Human Kidney Slide (Abnormal) (5 slides/pk)

HTS-10455 1 pk
EUR 286.00

Human Heart Slide (Normal) (5 slides/pk)

HTS-10501 1 pk
EUR 286.00

Human Heart Slide (Tumor) (5 slides/pk)

HTS-10502 1 pk
EUR 286.00

Human Heart Slide (Myxoma) (5 slides/pk)

HTS-10503 1 pk
EUR 286.00

Human Heart Slide (Abnormal) (5 slides/pk)

HTS-10525 1 pk
EUR 286.00

Human Prostate Tissue Slide (Hyperplasia) (5 slides/pk)

HTS-10635 1 pk
EUR 286.00

Human Colorectal Slide (Normal) (5 slides/pk)

HTS-10701 1 pk
EUR 286.00

Human Colorectal Slide (Benign) (5 slides/pk)

HTS-10702 1 pk
EUR 286.00

Human Colorectal Slide (Adenocarcinoma) (5 slides/pk)

HTS-10704 1 pk
EUR 286.00

Human Colorectal Colon Slide (Mucinous Carcinoma) (5 slides/pk)

HTS-10705 1 pk
EUR 286.00

Human Colorectal Colon Slide (Unclassified Carcinoma) (5 slides/pk)

HTS-10710 1 pk
EUR 286.00

Human Colorectal Colon Slide (Abnormal) (5 slides/pk)

HTS-10719 1 pk
EUR 286.00

Human Stomach Tissue Slide (Normal) (5 slides/pk)

HTS-10801 1 pk
EUR 286.00

Human Stomach Tissue Slide (Benign) (5 slides/pk)

HTS-10803 1 pk
EUR 286.00

Human Stomach Tissue Slide (Carcinoma) (5 slides/pk)

HTS-10807 1 pk
EUR 286.00

Human Stomach Tissue Slide (Adenocarcinoma) (5 slides/pk)

HTS-10809 1 pk
EUR 286.00

Human Stomach Tissue Slide (Mucinous adenocarcinoma) (5 slides/pk)

HTS-10810 1 pk
EUR 286.00

Human Stomach Tissue Slide (Signet Ring Cell Adenocarcinoma) (5 slides/pk)

HTS-10811 1 pk
EUR 286.00

Human Stomach Tissue Slide (Carcinoma) (5 slides/pk)

HTS-10814 1 pk
EUR 286.00

Human Stomach Tissue Slide (Abnormal) (5 slides/pk)

HTS-10826 1 pk
EUR 286.00

Human Stomach Tissue Slide (Tumor) (5 slides/pk)

HTS-10827 1 pk
EUR 286.00

Human Spleen Tissue Slide (Unknown) (5 slides/pk)

HTS-10900 1 pk
EUR 286.00

Human Spleen Tissue Slide (Normal) (5 slides/pk)

HTS-10901 1 pk
EUR 286.00

Human Spleen Tissue Slide (Benign) (5 slides/pk)

HTS-10903 1 pk
EUR 286.00

Human Thyroid Tissue Slide (Benign) (5 slides/pk)

HTS-11002 1 pk
EUR 286.00

Human Thyroid Tissue Slide (Follicular Carcinoma) (5 slides/pk)

HTS-11006 1 pk
EUR 286.00

Human Thyroid Tissue Slide (Papillary Carcinoma) (5 slides/pk)

HTS-11007 1 pk
EUR 286.00

Human Thyroid Tissue Slide (Abnormal) (5 slides/pk)

HTS-11018 1 pk
EUR 286.00

Human Pancreas Tissue Slide (normal) (5 slides/pk)

HTS-11101 1 pk
EUR 286.00

Human Pancreas Tissue Slide (Adenocarcinoma) (5 slides/pk)

HTS-11105 1 pk
EUR 286.00

Human Pancreas Tissue Slide (Abnormal) (5 slides/pk)

HTS-11106 1 pk
EUR 286.00

Human Ovary Tissue Slide (Normal) (5 slides/pk)

HTS-11201 1 pk
EUR 286.00

Human Ovary Tissue Slide (Benign) (5 slides/pk)

HTS-11202 1 pk
EUR 286.00

Human Ovary Tissue Slide (Benign Epithelial Tumor) (5 slides/pk)

HTS-11203 1 pk
EUR 286.00

Human Ovary Tissue Slide (Benign Sex Chord Stromal tumor) (5 slides/pk)

HTS-11204 1 pk
EUR 286.00

Human Ovary Tissue Slide (Malignant tumor) (5 slides/pk)

HTS-11206 1 pk
EUR 286.00

Human Ovary Tissue Slide (Epithelial tumor) (5 slides/pk)

HTS-11207 1 pk
EUR 286.00

Human Ovary Tissue Slide (Abnormal) (5 slides/pk)

HTS-11210 1 pk
EUR 286.00

Human Cervix Slide (Normal) (5 slides/pk)

HTS-11301 1 pk
EUR 286.00

Human Cervix Slide (Benign) (5 slides/pk)

HTS-11303 1 pk
EUR 286.00

Human Cervix Slide (Condyloma acuminatum) (5 slides/pk)

HTS-11304 1 pk
EUR 286.00

Human Cervix Slide (Interepithelial Neoplasia (squamous dysplasia)) (5 slides/pk)

HTS-11305 1 pk
EUR 286.00

Human Cervix Slide (Squamous Cell Carcinoma) (5 slides/pk)

HTS-11307 1 pk
EUR 286.00

Human Cervix Slide (Abnormal) (5 slides/pk)

HTS-11316 1 pk
EUR 286.00

Human Uterus Tissue Slide (Normal) (5 slides/pk)

HTS-11401 1 pk
EUR 286.00

Human Uterus Tissue Slide (Benign) (5 slides/pk)

HTS-11402 1 pk
EUR 286.00

Human Uterus Tissue Slide (Adenocarcinoma) (5 slides/pk)

HTS-11404 1 pk
EUR 286.00

Human Uterus Tissue Slide (Endometrioid Adenocarcinoma) (5 slides/pk)

HTS-11405 1 pk
EUR 286.00

Human Uterus Tissue Slide (Papillary serous Adenocarcinoma) (5 slides/pk)

HTS-11407 1 pk
EUR 286.00

Human Uterus Tissue Slide (Spindle cell tumor) (5 slides/pk)

HTS-11418 1 pk
EUR 286.00

Human Uterus Tissue Slide (Abnormal) (5 slides/pk)

HTS-11419 1 pk
EUR 286.00

Human Uterus Tissue Slide (Adenomyoma) (5 slides/pk)

HTS-11432 1 pk
EUR 286.00

Human Lymphoid Tissue Slide (Abnormal) (5 slides/pk)

HTS-11504 1 pk
EUR 286.00

Human Lymphoid Tissue Slide (Appendix Abnormal) (5 slides/pk)

HTS-11515 1 pk
EUR 286.00

Human Lymphoid Tissue Slide (Lymph node normal) (5 slides/pk)

HTS-11521 1 pk
EUR 286.00

Human Lymphoid Tissue Slide (Lymph node benign) (5 slides/pk)

HTS-11523 1 pk
EUR 286.00

Human Lymphoid Tissue Slide (Lymph node malignant) (5 slides/pk)

HTS-11524 1 pk
EUR 286.00

Human Lymphoid Tissue Slide (Lymph node abnormal) (5 slides/pk)

HTS-11525 1 pk
EUR 286.00

Human Lymphoid Tissue Slide (Tonsil benign) (5 slides/pk)

HTS-11533 1 pk
EUR 286.00

Human Leukemias/Lymphomas Tissue Slide (Leukemia Spleen) (5 slides/pk)

HTS-11601 1 pk
EUR 286.00

Human Leukemias/Lymphomas Tissue Slide (Lymphoma) (5 slides/pk)

HTS-11603 1 pk
EUR 286.00

Human Leukemias/Lymphomas Tissue Slide (Mixed Leukemia and Lymphoma) (5 slides/pk)

HTS-11606 1 pk
EUR 286.00

Human Testis Tissue Slide (Normal) (5 slides/pk)

HTS-11701 1 pk
EUR 286.00

Human Testis Tissue Slide (Abnormal) (5 slides/pk)

HTS-11710 1 pk
EUR 286.00

Human Small Intestine Tissue Slide (Squamous Cell Carcinoma) (5 slides/pk)

HTS-11801 1 pk
EUR 286.00

Human Small Intestine Tissue Slide (Squamous Cell Carcinoma) (5 slides/pk)

HTS-11814 1 pk
EUR 286.00

Human Small Intestine Tissue Slide (Squamous Cell Carcinoma) (5 slides/pk)

HTS-11815 1 pk
EUR 286.00

Human Small Intestine Tissue Slide (Squamous Cell Carcinoma) (5 slides/pk)

HTS-11824 1 pk
EUR 286.00

Human Urogenital Tissue Slide (Fallopian Tube normal) (5 slides/pk)

HTS-11901 1 pk
EUR 286.00

Human Urogenital Tissue Slide (Fallopian Tube Benign) (5 slides/pk)

HTS-11903 1 pk
EUR 286.00

Human Urogenital Tissue Slide (Fallopian Tube Abnormal) (5 slides/pk)

HTS-11905 1 pk
EUR 286.00

Human Urogenital Tissue Slide (Placenta Benign) (5 slides/pk)

HTS-11913 1 pk
EUR 286.00

Human Urogenital Tissue Slide (Vas Deferens Normal) (5 slides/pk)

HTS-11961 1 pk
EUR 286.00

Human Urogenital Tissue Slide (Vas Deferens Benign) (5 slides/pk)

HTS-11963 1 pk
EUR 286.00

Human Salivary Gland Tissue Slide (Mucoepidermoid carcinoma) (5 slides/pk)

HTS-12109 1 pk
EUR 286.00

Human Salivary Gland Tissue Slide (Abnormal) (5 slides/pk)

HTS-12128 1 pk
EUR 286.00

Human Penis Tissue Slide (Malignant) (5 slides/pk)

HTS-12204 1 pk
EUR 286.00

Human Soft Tissue Slide (Benign) (5 slides/pk)

HTS-12303 1 pk
EUR 286.00

Human Soft Tissue Slide (Abnormal) (5 slides/pk)

HTS-12305 1 pk
EUR 286.00

There is no limit to the number of genes that can be used to search the database in one query. secondary analysis such as gene ontology, regulatory factors, the location of the plot chromosome or tissue expression of IFN-regulated genes (IRGs) can be done in Interferome v2.0, or data can be downloaded in text format that is readily compatible with the common secondary analysis program. Given the importance of IFN to the innate immune response in infectious, inflammatory diseases and cancer, this upgrade of Interferome to version 2.0 will facilitate the identification of signatures of genes important in the pathogenesis of this disease.

Trevor

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