OBJECTIVE We combined gene expression and metabolic profile analysis to identify factors associated with the outcomes of patients with hepatocellular carcinoma (HCC).
METHOD We compared the pattern of metabolism and gene expression between paired tumor and nontumor tissue from 30 patients with HCC, and validated the results using a sample of 356 patients with HCC. A total of 469 metabolites were measured by using chromatography / mass spectrometry spectrometry liquid and gas chromatography / mass. Metabolism and integrated genomic data, and Kaplan-Meier and Cox proportional hazards analysis was used to a certain pattern associate with patient outcomes. related factors were evaluated for their effect on cancer cells in vitro and tumor formation in nude mice.
RESULTS We identified 28 metabolites and 169 genes associated with aggressive HCC. lipid metabolite-stearoyl-CoA desaturase (SCD) is associated with the aberrant activity of palmitate signal in aggressive HCC samples. The expression of gene products associated with these metabolites, including SCD, was independently associated with survival time and recurrence of tumors in test and validation sets.
The combined expression of SCD and α-fetoprotein associated with outcomes of patients with early-stage liver cancer. Levels of monounsaturated palmitic acid, the product of SCD activity, an increase in aggressive HCCS; monounsaturated palmitic acid increased migration and invasion of HCC cells cultured and colony formation by liver cancer cells. HCC cells expressed small interfering RNA against SCD decreased cell migration and the formation of colonies in culture and reduced tumorigenicity in mice.
CONCLUSION By using a combination of gene expression and metabolic profile analysis, we identified a network of lipogenic involving SCD and palmitic signal and associated with the development of HCC and patient outcomes. Microarray platform and data that has been submitted to the Gene Expression Omnibus at NCBI public database following the guidelines MIAME. accession numbers: GPL4700 (platform), and GSE6857 (sample).
Integrated metabolite and gene expression profiles identify lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes.
Interferome v2.0: updated database of interferon-regulated genes described.
Interferome v2.0 is an update of an earlier version of DB Interferome NAR published in the 2009 edition of the database. Much better computing infrastructure now allows more complex and faster queries, and supports more data sets of types I, II and III interferon (IFN) -treated cells, rat or human.
Quantitative, MIAME appropriate data is collected, carried out a thorough analysis, standardized, quantitative and statistical and then significant changes in gene expression are uploaded. a comprehensive manual collection of metadata i n v2.0 allows flexible, detailed searches capacity including the parameters: range double change, the type of IFN, concentration and time, and cell / tissue types.
JBScreen Classic 7 (MPD based)
M-CS-107L
MiTeGen
24 solutions
EUR 249
Description: JBScreen Classic 7 (MPD based)
JBScreen Classic 10 (Salt based)
M-CS-110L
MiTeGen
24 solutions
EUR 249
Description: JBScreen Classic 10 (Salt based)
JBScreen Classic 2 (PEG 4000 based)
M-CS-102L
MiTeGen
24 solutions
EUR 249
Description: JBScreen Classic 2 (PEG 4000 based)
JBScreen Classic 3 (PEG 4000+ based)
M-CS-103L
MiTeGen
24 solutions
EUR 249
Description: JBScreen Classic 3 (PEG 4000+ based)
JBScreen Classic 8 (MPD/Alcohol based)
M-CS-108L
MiTeGen
24 solutions
EUR 249
Description: JBScreen Classic 8 (MPD/Alcohol based)
JBScreen Classic HTS II L (1.7 ml per well) (Ammonium Sulfate, MPD, Alcohol and Salt based)
M-CS-202L
MiTeGen
96 solutions
EUR 377
Description: JBScreen Classic HTS II L (1.7 ml per well) (Ammonium Sulfate, MPD, Alcohol and Salt based)
JBScreen Classic 9 (Alcohol/Salt based)
M-CS-109L
MiTeGen
24 solutions
EUR 249
Description: JBScreen Classic 9 (Alcohol/Salt based)
JBScreen Classic 1 (PEG 400 to 3000 based)
M-CS-101L
MiTeGen
24 solutions
EUR 249
Description: JBScreen Classic 1 (PEG 400 to 3000 based)
JBScreen Classic 5 (PEG 8000 to 20000 based)
M-CS-105L
MiTeGen
24 solutions
EUR 249
Description: JBScreen Classic 5 (PEG 8000 to 20000 based)
JBScreen Classic 6 (Ammonium Sulfate based)
M-CS-106L
MiTeGen
24 solutions
EUR 249
Description: JBScreen Classic 6 (Ammonium Sulfate based)
JBScreen Classic 4 (PEG 5000 MME to 8000 based)
M-CS-104L
MiTeGen
24 solutions
EUR 249
Description: JBScreen Classic 4 (PEG 5000 MME to 8000 based)
JBScreen LCP HTS
M-CS-213L
MiTeGen
96 solutions
EUR 535
Description: JBScreen LCP HTS
JBScreen Detergents HTS
M-CS-525
MiTeGen
96 solutions
EUR 789
Description: JBScreen Detergents HTS
JBScreen Thermofluor SPECIFIC HTS
M-CS-333
MiTeGen
93 solutions
EUR 397
Description: JBScreen Thermofluor SPECIFIC HTS
JBScreen Thermofluor FUNDAMENT HTS
M-CS-332
MiTeGen
93 solutions
EUR 397
Description: JBScreen Thermofluor FUNDAMENT HTS
JBScreen Membrane 1 - 4 & JBScreen Detergents HTS (4 + 1 Kits)
M-CS-308
MiTeGen
4 + 1 Kits
EUR 2129
Description: JBScreen Membrane 1 - 4 & JBScreen Detergents HTS (4 + 1 Kits)
JBScreen Membrane HTS (1.7ml per well)
M-CS-310
MiTeGen
96 solutions
EUR 621
Description: JBScreen Membrane HTS (1.7ml per well)
JBScreen JCSG++ HTS L (1.7 ml per well)
M-CS-206L
MiTeGen
96 solutions
EUR 395
Description: JBScreen JCSG++ HTS L (1.7 ml per well)
JBScreen PACT++ HTS L (1.7 ml per well)
M-CS-207L
MiTeGen
96 solutions
EUR 395
Description: JBScreen PACT++ HTS L (1.7 ml per well)
JBScreen Plus HTS L (1.0 ml per well)
M-CS-507L
MiTeGen
96 solutions
EUR 883
Description: JBScreen Plus HTS L (1.0 ml per well)
JBScreen Basic HTS L (1.7 ml per well)
M-CS-203L
MiTeGen
96 solutions
EUR 377
Description: JBScreen Basic HTS L (1.7 ml per well)
JBScreen Kinase HTS L (1.7 ml per well)
M-CS-204L
MiTeGen
96 solutions
EUR 621
Description: JBScreen Kinase HTS L (1.7 ml per well)
JBScreen PEG/Salt HTS L (1.7 ml per well)
M-CS-205L
MiTeGen
96 solutions
EUR 395
Description: JBScreen PEG/Salt HTS L (1.7 ml per well)
JBScreen Nuc-Pro HTS L (1.7 ml per well)
M-CS-209L
MiTeGen
96 solutions
EUR 508
Description: JBScreen Nuc-Pro HTS L (1.7 ml per well)
JBScreen Pentaerythritol HTS L (1.7 ml per well)
M-CS-210L
MiTeGen
96 solutions
EUR 621
Description: JBScreen Pentaerythritol HTS L (1.7 ml per well)
JBScreen LCP
M-CS-340
MiTeGen
4 x 24 solutions
EUR 1165
Description: JBScreen LCP
JBScreen Buffers
M-CS-214
MiTeGen
84 solutions
EUR 395
Description: JBScreen Buffers
JBScreen JCSG++ 1
M-CS-151
MiTeGen
24 solutions
EUR 263
Description: JBScreen JCSG++ 1
JBScreen JCSG++ 2
M-CS-152
MiTeGen
24 solutions
EUR 263
Description: JBScreen JCSG++ 2
JBScreen JCSG++ 3
M-CS-153
MiTeGen
24 solutions
EUR 263
Description: JBScreen JCSG++ 3
JBScreen JCSG++ 4
M-CS-154
MiTeGen
24 solutions
EUR 263
Description: JBScreen JCSG++ 4
JBScreen PACT++ 1
M-CS-161
MiTeGen
24 solutions
EUR 263
Description: JBScreen PACT++ 1
JBScreen PACT++ 2
M-CS-162
MiTeGen
24 solutions
EUR 263
Description: JBScreen PACT++ 2
JBScreen PACT++ 3
M-CS-163
MiTeGen
24 solutions
EUR 263
Description: JBScreen PACT++ 3
JBScreen PACT++ 4
M-CS-164
MiTeGen
24 solutions
EUR 263
Description: JBScreen PACT++ 4
JBScreen Basic 1
M-CS-121
MiTeGen
24 solutions
EUR 249
Description: JBScreen Basic 1
JBScreen Basic 2
M-CS-122
MiTeGen
24 solutions
EUR 249
Description: JBScreen Basic 2
JBScreen Basic 3
M-CS-123
MiTeGen
24 solutions
EUR 249
Description: JBScreen Basic 3
JBScreen Basic 4
M-CS-124
MiTeGen
24 solutions
EUR 249
Description: JBScreen Basic 4
JBScreen Kinase 1
M-CS-131
MiTeGen
24 solutions
EUR 414
Description: JBScreen Kinase 1
JBScreen Kinase 2
M-CS-132
MiTeGen
24 solutions
EUR 414
Description: JBScreen Kinase 2
JBScreen Kinase 3
M-CS-133
MiTeGen
24 solutions
EUR 414
Description: JBScreen Kinase 3
JBScreen Kinase 4
M-CS-134
MiTeGen
24 solutions
EUR 414
Description: JBScreen Kinase 4
JBScreen Plus Salts
M-CS-503
MiTeGen
24 solutions
EUR 276
Description: JBScreen Plus Salts
JBScreen JCSG++ 1 - 4
M-CS-155
MiTeGen
4 Kits
EUR 858
Description: JBScreen JCSG++ 1 - 4
JBScreen PACT++ 1 - 4
M-CS-165
MiTeGen
4 Kits
EUR 858
Description: JBScreen PACT++ 1 - 4
JBScreen Detergents 1
M-CS-521
MiTeGen
24 solutions
EUR 263
Description: JBScreen Detergents 1
JBScreen Detergents 2
M-CS-522
MiTeGen
24 solutions
EUR 263
Description: JBScreen Detergents 2
JBScreen Detergents 3
M-CS-523
MiTeGen
24 solutions
EUR 263
Description: JBScreen Detergents 3
JBScreen Detergents 4
M-CS-524
MiTeGen
24 solutions
EUR 263
Description: JBScreen Detergents 4
JBScreen Basic 1 - 4
M-CS-125
MiTeGen
4 Kits
EUR 813
Description: JBScreen Basic 1 - 4
JBScreen PEG/Salt 1
M-CS-141
MiTeGen
24 solutions
EUR 263
Description: JBScreen PEG/Salt 1
JBScreen PEG/Salt 2
M-CS-142
MiTeGen
24 solutions
EUR 263
Description: JBScreen PEG/Salt 2
JBScreen PEG/Salt 3
M-CS-143
MiTeGen
24 solutions
EUR 263
Description: JBScreen PEG/Salt 3
JBScreen PEG/Salt 4
M-CS-144
MiTeGen
24 solutions
EUR 263
Description: JBScreen PEG/Salt 4
JBScreen Buffers Xtreme
M-CS-215
MiTeGen
84 solutions
EUR 395
Description: JBScreen Buffers Xtreme
JBScreen Plus Additives
M-CS-504
MiTeGen
24 solutions
EUR 276
Description: JBScreen Plus Additives
JBScreen Plus Volatiles
M-CS-505
MiTeGen
24 solutions
EUR 276
Description: JBScreen Plus Volatiles
JBScreen Plus Complete all 5 JBScreen Plus kits for a special price
M-CS-506
MiTeGen
5 Kits
EUR 1103
Description: JBScreen Plus Complete all 5 JBScreen Plus kits for a special price
JBScreen Kinase 1 - 4
M-CS-135
MiTeGen
4 Kits
EUR 1349
Description: JBScreen Kinase 1 - 4
JBScreen Nuc-Pro 1
M-CS-181
MiTeGen
24 solutions
EUR 414
Description: JBScreen Nuc-Pro 1
JBScreen Nuc-Pro 2
M-CS-182
MiTeGen
24 solutions
EUR 414
Description: JBScreen Nuc-Pro 2
JBScreen Nuc-Pro 3
M-CS-183
MiTeGen
24 solutions
EUR 414
Description: JBScreen Nuc-Pro 3
JBScreen Nuc-Pro 4
M-CS-184
MiTeGen
24 solutions
EUR 414
Description: JBScreen Nuc-Pro 4
JBScreen Plus Chaotropic
M-CS-502
MiTeGen
24 solutions
EUR 276
Description: JBScreen Plus Chaotropic
JBScreen Plus Kosmotropic
M-CS-501
MiTeGen
24 solutions
EUR 276
Description: JBScreen Plus Kosmotropic
JBScreen PEG/Salt 1 - 4
M-CS-145
MiTeGen
4 Kits
EUR 858
Description: JBScreen PEG/Salt 1 - 4
JBScreen Nuc-Pro 1 - 4
M-CS-185
MiTeGen
4 Kits
EUR 1349
Description: JBScreen Nuc-Pro 1 - 4
JBScreen Pentaerythritol 1-4
M-CS-195
MiTeGen
4 Kits
EUR 1349
Description: JBScreen Pentaerythritol 1-4
JBScreen Membrane 4 (MPD, & Salt based)
M-CS-304L
MiTeGen
24 solutions
EUR 414
Description: JBScreen Membrane 4 (MPD, & Salt based)
JBScreen Membrane 1-4 - All 4 kits
M-CS-309
MiTeGen
4 Kits
EUR 1349
Description: JBScreen Membrane 1-4 - All 4 kits
JBScreen Pentaerythritol 1 (PEP 426 based)
M-CS-191
MiTeGen
24 solutions
EUR 414
Description: JBScreen Pentaerythritol 1 (PEP 426 based)
JBScreen Pentaerythritol 2 (PEP 629 based)
M-CS-192
MiTeGen
24 solutions
EUR 414
Description: JBScreen Pentaerythritol 2 (PEP 629 based)
JBScreen Pentaerythritol 3 (PEE 270 based)
M-CS-193
MiTeGen
24 solutions
EUR 414
Description: JBScreen Pentaerythritol 3 (PEE 270 based)
JBScreen Pentaerythritol 4 (PEE 797 based)
M-CS-194
MiTeGen
24 solutions
EUR 414
Description: JBScreen Pentaerythritol 4 (PEE 797 based)
JBScreen Membrane 1 (PEG 400 to PEG 2000 MME based)
M-CS-301L
MiTeGen
24 solutions
EUR 414
Description: JBScreen Membrane 1 (PEG 400 to PEG 2000 MME based)
JBScreen Membrane 2 (PEG 2000 MME to PEG 10000 based)
M-CS-302L
MiTeGen
24 solutions
EUR 414
Description: JBScreen Membrane 2 (PEG 2000 MME to PEG 10000 based)
JBScreen Membrane 3 (Ammonium Sulfate, Alcohol and Salt based)
M-CS-303L
MiTeGen
24 solutions
EUR 414
Description: JBScreen Membrane 3 (Ammonium Sulfate, Alcohol and Salt based)
SafeView™ Classic
G108
ABM
1.0 ml
EUR 85.2
Classic 25 Necktube Core
TW-D024-9C16
MiTeGen
1 UNIT
EUR 55
Description: Classic 25 Necktube Core
WSE-5400-U Printgraph Classic
2305401
Atto
1unit
EUR 9438
Description: M ai n uni t , Cont r ol , U V
WSE-5400-UP Printgraph Classic
2305403
Atto
1unit
EUR 12178.8
Description: M ai n uni t , Cont r ol , U V, Ther mal Pr i nt er
Classic CRISPR sgRNA Synthesis Kit
K1252-25
Biovision
25 Reactions
EUR 526.8
Description: Classic CRISPR sgRNA Synthesis Kit
WSE-5400-CyP Printgraph Classic
2305407
Atto
1unit
EUR 12836.4
Description: M ai n uni t , Cont r ol , CyanL ED, Ther mal Pr i nt er
LCP Classic Sandwich Set (20 sets)
MLCP-CSS1-1
MiTeGen
20 SETS
EUR 393
Description: LCP Classic Sandwich Set (20 sets)
Classic 25 Liquid Dewar (25 Liters)
TW-LD25C
MiTeGen
25 liter
EUR 1305
Description: Classic 25 Liquid Dewar (25 Liters)
Worthington Industries Classic 25 Roller Base
TW-D024-8C02
MiTeGen
1 UNIT
EUR 350
Description: Worthington Industries Classic 25 Roller Base
Tube Bundle Special (Wizard: Classic 1,2,3,4; Wizard: Cryo 1,2)
M-R-10095402
MiTeGen
288 X 10 ml
EUR 2070
Description: Tube Bundle Special (Wizard: Classic 1,2,3,4; Wizard: Cryo 1,2)
Wizard Classic 1 Tubes (48 formulations 10 mL volumes)
M-R-1009530
MiTeGen
48 X 10 ml
EUR 374
Description: Wizard Classic 1 Tubes (48 formulations 10 mL volumes)
Wizard Classic 2 Tubes (48 formulations 10 mL volumes)
M-R-1009531
MiTeGen
49 X 10 ml
EUR 374
Description: Wizard Classic 2 Tubes (48 formulations 10 mL volumes)
Wizard Classic 3 Tubes (48 formulations 10 mL volumes)
M-R-1009533
MiTeGen
48 X 10 ml
EUR 374
Description: Wizard Classic 3 Tubes (48 formulations 10 mL volumes)
Wizard Classic 4 Tubes (48 formulations 10 mL volumes)
M-R-1009534
MiTeGen
48 X 10 ml
EUR 374
Description: Wizard Classic 4 Tubes (48 formulations 10 mL volumes)
Heating Element Assembly for BactiZapper Classic (B1000), 115V
B1000-RA
Benchmark Scientific
1 each
EUR 173.4
Heating Element Assembly for BactiZapper Classic (B1000-E), 230V
B1000-RA-E
Benchmark Scientific
1 PC
EUR 173.4
Wizard Classic 1 and 2 Tubes (Suite 96 formulations 10 mL volumes)
M-R-1009532
MiTeGen
96 X 10 ml
EUR 690
Description: Wizard Classic 1 and 2 Tubes (Suite 96 formulations 10 mL volumes)
Wizard Classic 3 and 4 Tubes (Suite 96 formulations 10 mL volumes)
M-R-1009535
MiTeGen
96 X 10 ml
EUR 690
Description: Wizard Classic 3 and 4 Tubes (Suite 96 formulations 10 mL volumes)
Wizard Block Bundle Special (Wizard: Classic 1/2, 3/4 & Wizard: Cryo 1/2)
M-R-1008657
MiTeGen
-
EUR 914
Description: Wizard Block Bundle Special (Wizard: Classic 1/2, 3/4 & Wizard: Cryo 1/2)
Shelved Puck holder, SPINE Pucks (SC3 Baskets) for Transport - Classic Canister Design
M-SP-SPH-1C
MiTeGen
1 UNIT
EUR 386
Description: Shelved Puck holder, SPINE Pucks (SC3 Baskets) for Transport - Classic Canister Design
Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)
M-R-1008647
MiTeGen
96 X 1.7 ml
EUR 316
Description: Wizard Classic 1 and 2 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)
Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)
M-R-1008648
MiTeGen
97 X 1.7 ml
EUR 316
Description: Wizard Classic 3 and 4 96 Well Matrix Block Plate (96 formulations 1.7 mL volumes in a 96-Well block plate)
LCP Classic Starter Kit
Contains:
20 LCP Classic Sandwich Sets (MLCP-CSS1-1)
1 10 μL Syringe (for manual dispensing) (MLCP-10muSYR-1)
2 100 μL Syringe (for mixing) (MLCP-100muSYR-1)
1 Manual Dispenser (MLCP-PB600-1)
1 Symmetrical Mixer Union for LCP (MCLP
MLCP-CSK-1
MiTeGen
1 KIT
EUR 1993
Description: LCP Classic Starter KitContains:20 LCP Classic Sandwich Sets (MLCP-CSS1-1)1 10 μL Syringe (for manual dispensing) (MLCP-10muSYR-1)2 100 μL Syringe (for mixing) (MLCP-100muSYR-1)1 Manual Dispenser (MLCP-PB600-1)1 Symmetrical Mixer Union for LCP (MCLP-SMXU-1)6 short 22 gauge small hub needles (MLCP-HN22-6)1 Monoolien-9, 1g (MLCP-RMO9-1)1 4″ Soft Rubber Brayer (M-CTP-BRY1)1 Wizard Cubic LCP Screen (MLCP-RWCS-1)1 Box (20) MicroMounts: (M2-L18SP-A1)1 Box (20) MicroMounts: (M2-L18SP-A2)40 Reusable bases model B1A-R (GB-B1A-R-40)1 pair serrated end tweezers (TW-1)1 Carbide Scribe (MLCP-CBSC-1)5 replacement scribe tips (MLCP-CBT5)
Individual Jena Bioscience Screen Condition, 100 ml (Classic, Basic, Membrane, Cryo, Kinase, PEG/Salt) Indicate screen and condition # when placing the order
M-CS-IND-100ML
MiTeGen
100 ml
EUR 239
Description: Individual Jena Bioscience Screen Condition, 100 ml (Classic, Basic, Membrane, Cryo, Kinase, PEG/Salt) Indicate screen and condition # when placing the order
JBS Solubility Kit HTS
M-CO-311
MiTeGen
95 solutions
EUR 482
Description: JBS Solubility Kit HTS
Classical swine fever virus PCR kit
PCR-V130-48R
Bioingentech
50T
EUR 987.6
Classical swine fever virus PCR kit
PCR-V130-96R
Bioingentech
100T
EUR 1335.6
There is no limit to the number of genes that can be used to search the database in one query. secondary analysis such as gene ontology, regulatory factors, the location of the plot chromosome or tissue expression of IFN-regulated genes (IRGs) can be done in Interferome v2.0, or data can be downloaded in text format that is readily compatible with the common secondary analysi s program. Given the importance of IFN to the innate immune response in infectious, inflammatory diseases and cancer, this upgrade of Interferome to version 2.0 will facilitate the identification of signatures of genes important in the pathogenesis of this disease.
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