Integrated metabolite and gene expression profiles identify lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes.

Integrated metabolite and gene expression profiles identify lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes.

OBJECTIVE
We combined gene expression and metabolic profile analysis to identify factors associated with the outcomes of patients with hepatocellular carcinoma (HCC).


METHOD
We compared the pattern of metabolism and gene expression between paired tumor and nontumor tissue from 30 patients with HCC, and validated the results using a sample of 356 patients with HCC. A total of 469 metabolites were measured by using chromatography / mass spectrometry spectrometry liquid and gas chromatography / mass. Metabolism and integrated genomic data, and Kaplan-Meier and Cox proportional hazards analysis was used to a certain pattern associate with patient outcomes. related factors were evaluated for their effect on cancer cells in vitro and tumor formation in nude mice.


RESULTS
We identified 28 metabolites and 169 genes associated with aggressive HCC. lipid metabolite-stearoyl-CoA desaturase (SCD) is associated with the aberrant activity of palmitate signal in aggressive HCC samples. The expression of gene products associated with these metabolites, including SCD, was independently associated with survival time and recurrence of tumors in test and validation sets.

The combined expression of SCD and α-fetoprotein associated with outcomes of patients with early-stage liver cancer. Levels of monounsaturated palmitic acid, the product of SCD activity, an increase in aggressive HCCS; monounsaturated palmitic acid increased migration and invasion of HCC cells cultured and colony formation by liver cancer cells. HCC cells expressed small interfering RNA against SCD decreased cell migration and the formation of colonies in culture and reduced tumorigenicity in mice.


CONCLUSION
By using a combination of gene expression and metabolic profile analysis, we identified a network of lipogenic involving SCD and palmitic signal and associated with the development of HCC and patient outcomes. Microarray platform and data that has been submitted to the Gene Expression Omnibus at NCBI public database following the guidelines MIAME. accession numbers: GPL4700 (platform), and GSE6857 (sample).

Integrated metabolite and gene expression profiles identify lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes.
Integrated metabolite and gene expression profiles identify lipid biomarkers associated with progression of hepatocellular carcinoma and patient outcomes.

Interferome v2.0: updated database of interferon-regulated genes described.

Interferome v2.0 is an update of an earlier version of DB Interferome NAR published in the 2009 edition of the database. Much better computing infrastructure now allows more complex and faster queries, and supports more data sets of types I, II and III interferon (IFN) -treated cells, rat or human.

Quantitative, MIAME appropriate data is collected, carried out a thorough analysis, standardized, quantitative and statistical and then significant changes in gene expression are uploaded. a comprehensive manual collection of metadata in v2.0 allows flexible, detailed searches capacity including the parameters: range double change, the type of IFN, concentration and time, and cell / tissue types.

JBScreen Classic 2

CS-102L 24solutions
EUR 217.8

JBScreen Classic 3

CS-103L 24solutions
EUR 217.8

JBScreen Classic 4

CS-104L 24solutions
EUR 217.8

JBScreen Classic 5

CS-105L 24solutions
EUR 217.8

JBScreen Classic 6

CS-106L 24solutions
EUR 217.8

JBScreen Classic 7

CS-107L 24solutions
EUR 217.8

JBScreen Classic 8

CS-108L 24solutions
EUR 217.8

JBScreen Classic 9

CS-109L 24solutions
EUR 217.8

JBScreen Classic 10

CS-110L 24solutions
EUR 217.8

JBScreen Classic 1 - 5

CS-112L 5Kits
EUR 870

JBScreen Classic 6 - 10

CS-113L 5Kits
EUR 870

JBScreen Classic 1 - 10

CS-114L 10Kits
EUR 1611.8

JBScreen Classic 1 - 5

M-CS-112L 5 Kits
EUR 1082
Description: JBScreen Classic 1 - 5

JBScreen Classic 6 - 10

M-CS-113L 5 Kits
EUR 1082
Description: JBScreen Classic 6 - 10

JBScreen Classic 1 - 10

M-CS-114L 10 Kits
EUR 2005
Description: JBScreen Classic 1 - 10

JBScreen Classic 7 (MPD based)

M-CS-107L 24 solutions
EUR 249
Description: JBScreen Classic 7 (MPD based)

JBScreen Classic 10 (Salt based)

M-CS-110L 24 solutions
EUR 249
Description: JBScreen Classic 10 (Salt based)

JBScreen Classic HTS II L (1.7 ml per well) (Ammonium Sulfate, MPD, Alcohol and Salt based)

M-CS-202L 96 solutions
EUR 377
Description: JBScreen Classic HTS II L (1.7 ml per well) (Ammonium Sulfate, MPD, Alcohol and Salt based)

JBScreen Classic 2 (PEG 4000 based)

M-CS-102L 24 solutions
EUR 249
Description: JBScreen Classic 2 (PEG 4000 based)

JBScreen Classic 3 (PEG 4000+ based)

M-CS-103L 24 solutions
EUR 249
Description: JBScreen Classic 3 (PEG 4000+ based)

JBScreen Classic 8 (MPD/Alcohol based)

M-CS-108L 24 solutions
EUR 249
Description: JBScreen Classic 8 (MPD/Alcohol based)

JBScreen Classic 9 (Alcohol/Salt based)

M-CS-109L 24 solutions
EUR 249
Description: JBScreen Classic 9 (Alcohol/Salt based)

JBScreen Classic 6 (Ammonium Sulfate based)

M-CS-106L 24 solutions
EUR 249
Description: JBScreen Classic 6 (Ammonium Sulfate based)

JBScreen Classic 1 (PEG 400 to 3000 based)

M-CS-101L 24 solutions
EUR 249
Description: JBScreen Classic 1 (PEG 400 to 3000 based)

JBScreen Classic 5 (PEG 8000 to 20000 based)

M-CS-105L 24 solutions
EUR 249
Description: JBScreen Classic 5 (PEG 8000 to 20000 based)

JBScreen Classic 4 (PEG 5000 MME to 8000 based)

M-CS-104L 24 solutions
EUR 249
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JBScreen LCP HTS

CS-213L 96solutions
EUR 467.6

JBScreen LCP HTS

M-CS-213L 96 solutions
EUR 535
Description: JBScreen LCP HTS

JBScreen JCSG++ HTS

CS-206L 96solutions
EUR 345

JBScreen PACT++ HTS

CS-207L 96solutions
EUR 345

JBScreen Plus HTS

CS-507L 96solutions
EUR 771.6

JBScreen Basic HTS

CS-203L 96solutions
EUR 329.4

JBScreen Kinase HTS

CS-204L 96solutions
EUR 542.8

JBScreen Detergents HTS

M-CS-525 96 solutions
EUR 789
Description: JBScreen Detergents HTS

JBScreen Pentaerythritol HTS

CS-210L 96solutions
EUR 542.8

JBScreen PEG/Salt HTS

CS-205L 96solutions
EUR 345

JBScreen Nuc-Pro HTS

CS-209L 96solutions
EUR 543

JBScreen Wizard 1 & 2 HTS

CS-318 96solutions
EUR 329.4

JBScreen Thermofluor SPECIFIC HTS

M-CS-333 93 solutions
EUR 397
Description: JBScreen Thermofluor SPECIFIC HTS

JBScreen Thermofluor FUNDAMENT HTS

M-CS-332 93 solutions
EUR 397
Description: JBScreen Thermofluor FUNDAMENT HTS

JBScreen Membrane 1 - 4 & JBScreen Detergents HTS (4 + 1 Kits)

M-CS-308 4 + 1 Kits
EUR 2129
Description: JBScreen Membrane 1 - 4 & JBScreen Detergents HTS (4 + 1 Kits)

JBScreen Membrane HTS (1.7ml per well)

M-CS-310 96 solutions
EUR 621
Description: JBScreen Membrane HTS (1.7ml per well)

JBScreen JCSG++ HTS L (1.7 ml per well)

M-CS-206L 96 solutions
EUR 395
Description: JBScreen JCSG++ HTS L (1.7 ml per well)

JBScreen PACT++ HTS L (1.7 ml per well)

M-CS-207L 96 solutions
EUR 395
Description: JBScreen PACT++ HTS L (1.7 ml per well)

JBScreen Plus HTS L (1.0 ml per well)

M-CS-507L 96 solutions
EUR 883
Description: JBScreen Plus HTS L (1.0 ml per well)

JBScreen Basic HTS L (1.7 ml per well)

M-CS-203L 96 solutions
EUR 377
Description: JBScreen Basic HTS L (1.7 ml per well)

JBScreen Kinase HTS L (1.7 ml per well)

M-CS-204L 96 solutions
EUR 621
Description: JBScreen Kinase HTS L (1.7 ml per well)

JBScreen PEG/Salt HTS L (1.7 ml per well)

M-CS-205L 96 solutions
EUR 395
Description: JBScreen PEG/Salt HTS L (1.7 ml per well)

JBScreen Nuc-Pro HTS L (1.7 ml per well)

M-CS-209L 96 solutions
EUR 508
Description: JBScreen Nuc-Pro HTS L (1.7 ml per well)

JBScreen Pentaerythritol HTS L (1.7 ml per well)

M-CS-210L 96 solutions
EUR 621
Description: JBScreen Pentaerythritol HTS L (1.7 ml per well)

JBScreen LCP

CS-340 4 x 24solutions
EUR 936.3

JBScreen LCP

M-CS-340 4 x 24 solutions
EUR 1165
Description: JBScreen LCP

JBScreen Buffers

CS-214 84solutions
EUR 345

JBScreen Buffers

M-CS-214 84 solutions
EUR 395
Description: JBScreen Buffers

JBScreen JCSG++ 1

CS-151 24solutions
EUR 229.9

JBScreen JCSG++ 2

CS-152 24solutions
EUR 229.9

JBScreen JCSG++ 3

CS-153 24solutions
EUR 229.9

JBScreen JCSG++ 4

CS-154 24solutions
EUR 229.9

JBScreen PACT++ 1

CS-161 24solutions
EUR 229.9

JBScreen PACT++ 2

CS-162 24solutions
EUR 229.9

JBScreen PACT++ 3

CS-163 24solutions
EUR 229.9

JBScreen PACT++ 4

CS-164 24solutions
EUR 229.9

JBScreen JCSG++ 1

M-CS-151 24 solutions
EUR 263
Description: JBScreen JCSG++ 1

JBScreen JCSG++ 2

M-CS-152 24 solutions
EUR 263
Description: JBScreen JCSG++ 2

JBScreen JCSG++ 3

M-CS-153 24 solutions
EUR 263
Description: JBScreen JCSG++ 3

JBScreen JCSG++ 4

M-CS-154 24 solutions
EUR 263
Description: JBScreen JCSG++ 4

JBScreen PACT++ 1

M-CS-161 24 solutions
EUR 263
Description: JBScreen PACT++ 1

JBScreen PACT++ 2

M-CS-162 24 solutions
EUR 263
Description: JBScreen PACT++ 2

JBScreen PACT++ 3

M-CS-163 24 solutions
EUR 263
Description: JBScreen PACT++ 3

JBScreen PACT++ 4

M-CS-164 24 solutions
EUR 263
Description: JBScreen PACT++ 4

JBScreen Basic 1

CS-121 24solutions
EUR 217.8

JBScreen Basic 2

CS-122 24solutions
EUR 217.8

JBScreen Basic 3

CS-123 24solutions
EUR 217.8

JBScreen Basic 4

CS-124 24solutions
EUR 217.8

JBScreen Basic 1

M-CS-121 24 solutions
EUR 249
Description: JBScreen Basic 1

JBScreen Basic 2

M-CS-122 24 solutions
EUR 249
Description: JBScreen Basic 2

JBScreen Basic 3

M-CS-123 24 solutions
EUR 249
Description: JBScreen Basic 3

JBScreen Basic 4

M-CS-124 24 solutions
EUR 249
Description: JBScreen Basic 4

JBScreen Kinase 1

CS-131 24solutions
EUR 361.5

JBScreen Kinase 2

CS-132 24solutions
EUR 361.5

JBScreen Kinase 3

CS-133 24solutions
EUR 361.5

JBScreen Kinase 4

CS-134 24solutions
EUR 361.5

JBScreen Wizard 1

CS-311 48solutions
EUR 435.6

JBScreen Wizard 2

CS-312 48solutions
EUR 435.6

JBScreen Kinase 1

M-CS-131 24 solutions
EUR 414
Description: JBScreen Kinase 1

JBScreen Kinase 2

M-CS-132 24 solutions
EUR 414
Description: JBScreen Kinase 2

JBScreen Kinase 3

M-CS-133 24 solutions
EUR 414
Description: JBScreen Kinase 3

JBScreen Kinase 4

M-CS-134 24 solutions
EUR 414
Description: JBScreen Kinase 4

JBScreen Plus Salts

CS-503 24solutions
EUR 241

JBScreen Plus Salts

M-CS-503 24 solutions
EUR 276
Description: JBScreen Plus Salts

JBScreen Membrane 1

CS-301L 24solutions
EUR 361.5

JBScreen Membrane 2

CS-302L 24solutions
EUR 361.5

JBScreen Membrane 3

CS-303L 24solutions
EUR 361.5

JBScreen Membrane 4

CS-304L 24solutions
EUR 361.5

JBScreen Plus Complete all 5 JBScreen Plus kits for a special price

M-CS-506 5 Kits
EUR 1103
Description: JBScreen Plus Complete all 5 JBScreen Plus kits for a special price

JBScreen Plus Complete

CS-506 5Kits
EUR 963.9

JBScreen Detergents 1

M-CS-521 24 solutions
EUR 263
Description: JBScreen Detergents 1

JBScreen Detergents 2

M-CS-522 24 solutions
EUR 263
Description: JBScreen Detergents 2

JBScreen Detergents 3

M-CS-523 24 solutions
EUR 263
Description: JBScreen Detergents 3

JBScreen Detergents 4

M-CS-524 24 solutions
EUR 263
Description: JBScreen Detergents 4

JBScreen JCSG++ 1 - 4

CS-155 4Kits
EUR 689.8

JBScreen PACT++ 1 - 4

CS-165 4Kits
EUR 689.8

JBScreen JCSG++ 1 - 4

M-CS-155 4 Kits
EUR 858
Description: JBScreen JCSG++ 1 - 4

JBScreen PACT++ 1 - 4

M-CS-165 4 Kits
EUR 858
Description: JBScreen PACT++ 1 - 4

JBScreen Buffers Xtreme

CS-215 84solutions
EUR 345

JBScreen Plus Additives

CS-504 24solutions
EUR 241

JBScreen Plus Volatiles

CS-505 24solutions
EUR 241

JBScreen Buffers Xtreme

M-CS-215 84 solutions
EUR 395
Description: JBScreen Buffers Xtreme

JBScreen Plus Additives

M-CS-504 24 solutions
EUR 276
Description: JBScreen Plus Additives

JBScreen Plus Volatiles

M-CS-505 24 solutions
EUR 276
Description: JBScreen Plus Volatiles

JBScreen Basic 1 - 4

CS-125 4Kits
EUR 653.4

JBScreen PEG/Salt 1

CS-141 24solutions
EUR 229.9

JBScreen PEG/Salt 2

CS-142 24solutions
EUR 229.9

JBScreen PEG/Salt 3

CS-143 24solutions
EUR 229.9

JBScreen PEG/Salt 4

CS-144 24solutions
EUR 229.9

JBScreen Basic 1 - 4

M-CS-125 4 Kits
EUR 813
Description: JBScreen Basic 1 - 4

JBScreen PEG/Salt 1

M-CS-141 24 solutions
EUR 263
Description: JBScreen PEG/Salt 1

JBScreen PEG/Salt 2

M-CS-142 24 solutions
EUR 263
Description: JBScreen PEG/Salt 2

JBScreen PEG/Salt 3

M-CS-143 24 solutions
EUR 263
Description: JBScreen PEG/Salt 3

JBScreen PEG/Salt 4

M-CS-144 24 solutions
EUR 263
Description: JBScreen PEG/Salt 4

JBScreen Plus Chaotropic

M-CS-502 24 solutions
EUR 276
Description: JBScreen Plus Chaotropic

JBScreen Kinase 1 - 4

CS-135 4Kits
EUR 1084.5

JBScreen Nuc-Pro 1

CS-181 24solutions
EUR 362

JBScreen Nuc-Pro 2

CS-182 24solutions
EUR 362

JBScreen Nuc-Pro 3

CS-183 24solutions
EUR 362

JBScreen Nuc-Pro 4

CS-184 24solutions
EUR 362

JBScreen Wizard 1 & 2

CS-315 2Kits
EUR 826.9

JBScreen Kinase 1 - 4

M-CS-135 4 Kits
EUR 1349
Description: JBScreen Kinase 1 - 4

JBScreen Nuc-Pro 1

M-CS-181 24 solutions
EUR 414
Description: JBScreen Nuc-Pro 1

JBScreen Nuc-Pro 2

M-CS-182 24 solutions
EUR 414
Description: JBScreen Nuc-Pro 2

JBScreen Nuc-Pro 3

M-CS-183 24 solutions
EUR 414
Description: JBScreen Nuc-Pro 3

JBScreen Nuc-Pro 4

M-CS-184 24 solutions
EUR 414
Description: JBScreen Nuc-Pro 4

JBScreen Plus Kosmotropic

CS-501 24solutions
EUR 241

JBScreen Plus Kosmotropic

M-CS-501 24 solutions
EUR 276
Description: JBScreen Plus Kosmotropic

JBScreen Membrane 1 - 4

CS-309 4Kits
EUR 1084.5

JBScreen Pentaerythritol 1

CS-191 24solutions
EUR 361.5

JBScreen Pentaerythritol 2

CS-192 24solutions
EUR 361.5

JBScreen Pentaerythritol 3

CS-193 24solutions
EUR 361.5

JBScreen Pentaerythritol 4

CS-194 24solutions
EUR 361.5

JBScreen Thermofluor SPECIFIC

CS-333 93solutions
EUR 347.1

JBScreen PEG/Salt 1 - 4

CS-145 4Kits
EUR 689.8

JBScreen PEG/Salt 1 - 4

M-CS-145 4 Kits
EUR 858
Description: JBScreen PEG/Salt 1 - 4

JBScreen Nuc-Pro 1 - 4

CS-185 4Kits
EUR 1085

JBScreen Nuc-Pro 1 - 4

M-CS-185 4 Kits
EUR 1349
Description: JBScreen Nuc-Pro 1 - 4

JBScreen Pentaerythritol 1 - 4

CS-195 4Kits
EUR 1084.5

JBScreen Pentaerythritol 1-4

M-CS-195 4 Kits
EUR 1349
Description: JBScreen Pentaerythritol 1-4

JBScreen Membrane 4 (MPD, & Salt based)

M-CS-304L 24 solutions
EUR 414
Description: JBScreen Membrane 4 (MPD, & Salt based)

JBScreen Membrane 1-4 - All 4 kits

M-CS-309 4 Kits
EUR 1349
Description: JBScreen Membrane 1-4 - All 4 kits

JBScreen Pentaerythritol 1 (PEP 426 based)

M-CS-191 24 solutions
EUR 414
Description: JBScreen Pentaerythritol 1 (PEP 426 based)

JBScreen Pentaerythritol 2 (PEP 629 based)

M-CS-192 24 solutions
EUR 414
Description: JBScreen Pentaerythritol 2 (PEP 629 based)

JBScreen Pentaerythritol 3 (PEE 270 based)

M-CS-193 24 solutions
EUR 414
Description: JBScreen Pentaerythritol 3 (PEE 270 based)

There is no limit to the number of genes that can be used to search the database in one query. secondary analysis such as gene ontology, regulatory factors, the location of the plot chromosome or tissue expression of IFN-regulated genes (IRGs) can be done in Interferome v2.0, or data can be downloaded in text format that is readily compatible with the common secondary analysis program. Given the importance of IFN to the innate immune response in infectious, inflammatory diseases and cancer, this upgrade of Interferome to version 2.0 will facilitate the identification of signatures of genes important in the pathogenesis of this disease.

Trevor

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