BLASTX and PIR_NREF FUNGEN Pine EST database of Sorgum sequences cDNA inserts ASGR-PPCR
Reproducibility and Reuse of Adaptive Immune Receptor Repertoire Data.
High-throughput sequencing (HTS) immunoglobulin (B cell receptors, antibodies) and the T-cell receptor repertoire has increased dramatically since the technique was introduced in 2009 (1-3). This experimental approach discusses the maturation of the adaptive immune system and its response to antigens, pathogens and disease conditions in exquisite detail. It holds significant promise for diagnostic and therapeutic applications-guiding. New technologies often spread rapidly, sometimes faster than an understanding of how to create technology products that are reliable, reproduced, or used by others.
As a complex technology has been developed, the scientific community has come together to adopt common standards, protocols and policies to generate and share data sets, such as MIAME developed protocols for microarray experiments. Adaptive Immune Receptor Repertoire (AIRR) Community formed in 2015 to address the same issue for HTS Data immune repertoire. The purpose of this perspective is to provide an overview of the principles of Community AIRR establishment and presents the progress that AIRR Community has made in developing a protocol standard practices and share data.
Finally, and most importantly, we invite all interested parties to join this effort to facilitate the distribution and use of robust data sets Calophyllum Brasiliense (Calophyllaceae) is a tropical rain forest trees, mainly distributed in South and Central America. It is an important source of natural bioactive products such as, for example soulatrolide, and the type of mammea coumarin. Soulatrolide is dipyranocoumarins tetracyclic and potent inhibitor of HIV-1 reverse transcriptase and Mycobacterium tuberculosis. Mammea A / BA and A / BB coumarin, pure or as a mixture, it is active against several leukemia cell lines, Trypanosoma cruzi and Leishmania amazonensis.
In this study, analysis of toxicogenomic of Soulatrolide and Mammea A / BA + A / B (3: 1) mixture is carried out to validate the potential toxicology of types of compounds. Soulatrolide or mixture mammea A / BA + A / B (3: 1) was orally administered to male mice (CD-1) at a dose of 100 mg / kg / day for 1 week. After this time, the rats were sacrificed and RNA was extracted from the liver of animals treated. Transcriptomic analysis performed using Affymetrix Mouse Gene 1.0 ST Array.
Strong microarray analysis (RMA) and two-way ANOVA revealed for the treatment of mixtures mammea 46 genes upregulated and 72 genes downregulated; Meanwhile, for soulatrolide 665 upregulated and downregulated genes 1077. enrichment analysis of these genes revealed that in both types of treatment of genetic expression is mainly involved in drug metabolism. Overall results demonstrate the safety profile. Adhered to the guidelines MIAME microarray data and stored in GEO with accession numbers
Reproducibility and Reuse of Adaptive Immune Receptor Repertoire Data.
Gene expression profiles of Brakeless mutant Drosophila embryos.
The transcriptional co-regulator Brakeless perform many important functions during Drosophila development, but few target genes have been identified.
Here we use the Affymetrix microarray to identify the genes Brakeless-regulated in Drosophila embryos 2-4 hours old. Strong analytical multi-array (RMA) and the statistical test revealed 240 genes that change their expression is more than 1.5 times.
Description: The OxiSelect Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The kit?s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples.
Description: The OxiSelect Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The kit?s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples.
Description: Cell Biolabs? OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume).
Description: Cell Biolabs? OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume).
Description: Cell Biolabs? OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume).
Description: Cell Biolabs? OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume).
Description: Cell Biolabs? Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate. The kit can be also used in culturing cells in 3D collagen matrix.
Description: Indirect Colorimetric assay used for quantitative measuring Nitrate and Nitrite present in a variety of samples in Serum, Plasma, Urine, Saliva, Water, Buffer, Cell Lysates, Tissue Culture Media samples from all species
Description: A competitive ELISA for quantitative measurement of Goat Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Nitric oxide synthase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Histamine is naturally occurring in food, with high concentrations associated with spoiled and fermented foods. Exposure to high levels of histamine through the ingestion of food can cause symptoms similar to an allergic response. Our Histamine Assay Kit detects total histamine from food samples using a colorimetric probe. Reduction of the probe yields color development proportional the histamine levels in the sample. Absorbance at 450nm is read after a one hour incubation at 37C and histamine levels are calculated based on a histamine standard curve.
Description: The Lactose Assay Kit measures total lactose in milk based food products or biological samples such as blood or urine. Lactose is cleaved into glucose and galactose. Glucose is then oxidized, yielding hydrogen peroxide and D-gluconic acid. The hydrogen peroxide is detected by a fluorometric probe.
Description: Bilirubin, a byproduct of heme breakdown, can exist conjugated to glucuronic acid (direct) and as unconjugated (indirect). The unconjugated form is found in the blood bound to albumin and is transported to the liver. Bilirubin becomes conjugated to glucuronic acid in the liver, making it more soluble and allowing for excretion into bile. High levels of bilirubin have been correlated with jaundice and Gilbert?s syndrome while low levels have been associated with cardiovascular disease and diabetes mellitus.
Description: Our Pyruvate Assay Kit measures pyruvate in biological samples. First, pyruvate is oxidized by pyruvate oxidase, producing hydrogen peroxide. The hydrogen peroxide is then detected at ex. 530-570 nm/em. 590-600 nm using a specific fluorometric probe. Pyruvate levels in unknown samples are determined based on the provided pyruvate standard curve.
Description: Cell Biolabs? Urea Assay Kit is based on the Berthelot reaction. Urea is first degraded into ammonia and carbon dioxide, which further reacts with an alkaline developer to produce a blue-green colored product that can be measured with a standard spectrophotometric plate reader at an optical density between 580-630 nm. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, urea standards and unknown samples.
Description: Cell Biolabs? Phosphatidylcholine Assay Kit measures the phosphatidylcholine present within serum, plasma, or tissue samples.Samples are compared to a known concentration of phosphatidylcholine standard within the 96-well microtiter plate format. Samples and standards are incubated for 60 minutes and then read with a standard 96-well fluorometric plate reader.
Description: Cell Biolabs? Sphingomyelin Assay Kit is a simple fluorometric assay that measures the amount of sphingomyelin present in plasma or serum, tissue homogenates, or cell suspensionsin a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, sphingomyelin standards and unknown samples. Sample sphingomyelin concentrations are determined by comparison with a known sphingomyelin standard.
Description: Glutamate is a non-essential amino acid that serves as an important neurotransmitter in the mammalian brain and has a key role in cellular metabolism. Excess glutamate levels in the brain can cause cell injury and death, leading to neurological diseases. Our Glutamate Assay Kit is a quantitative, fluorometric assay that uses glutamate specific enzymes to generate hydrogen peroxide. An ADHP probe is oxidized by hydrogen peroxide to generate fluorescent Resorufin, which correlates to the level of glutamate in the sample. Glutamate levels in an unknown sample are calculated based on a glutamate standard curve.
Description: The Hydroxyproline Assay Kit is a quantitative colorimetric assay for measuring the hydroxyproline concentration in protein samples, including collagen where it is found almost exclusively.
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat.
Description: Cell Biolabs? Cell Contraction Assays (Floating Matrix Model) provide a simple, in vitro system to assess cell contractivity and screen cell contraction mediators. The proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction.
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation. The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1). An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.
Description: Our Lactate Assay Kit measures L-lactate in biological samples. Lactate is first oxidized by lactate oxidase, yielding pyruvate and hydrogen peroxide. The hydrogen peroxide released from this reaction is specifically detected by either a colorimetric or fluorometric probe in a 1:1 ratio. Lactate levels in unknown samples are determined based on a lactate standard curve.
Description: Our Lactate Assay Kit measures L-lactate in biological samples. Lactate is first oxidized by lactate oxidase, yielding pyruvate and hydrogen peroxide. The hydrogen peroxide released from this reaction is specifically detected by either a colorimetric or fluorometric probe in a 1:1 ratio. Lactate levels in unknown samples are determined based on a lactate standard curve.
Description: Our NAD+/NADH Assay Kit detects NAD+ and NADH in cell and tissue lysates. Total NAD+/NADH can be detected or samples can be treated with an acid or base treatment to specifically detect NAD+ or NADH. This assay uses an enzymatic cycling reaction that reduces NAD+ to NADH, which then reacts with a colorimetric probe and is detected with a spectrophotometric plate reader at 450nm. NAD+/NADH levels in unknown samples are calculated based on the provided NAD+ standard curve.
Description: Our Soluble Collagen Assay Kit detects soluble collagen in cell and tissue lysates. This assay uses a Sirius Red Reagent to stain the triple helix structure of collagen that has been dried onto the wells of a 96-well plate. The collagen is washed with an Acidic Reagent and the stain is eluted with a Basic Reagent, which is then transferred to a new plate and read colorimetrically between 540nm-560nm. The amount of soluble collagen in samples is determined based on a provided collagen standard curve.
Description: Our BCG Albumin Assay utilizes a proprietary bromocresol green (BCG) formulation for the detection of albumin in biological samples. BCG forms a color complex after 5 minutes when bound to albumin and is detected colorimetrically between 570-670nm. The amount of albumin in samples is determined based on a provided albumin standard curve.
Description: Our NADP+/NADPH Assay Kit detects NADP+ and NADPH in cell and tissue lysates. Total NADP+/NADPH can be detected or samples can be treated with an acid or base treatment to specifically detect NADP+ or NADPH. This assay uses an enzymatic cycling reaction that reduces NADP+ to NADPH, which then reacts with a colorimetric probe and is detected with a spectrophotometric plate reader at 450nm. NADP+/ NADPH levels in unknown samples are calculated based on the provided NADP+ standard curve.
Description: Our Glycogen Assay Kit (Fluorometric) measures glycogen in serum, plasma, urine, lysates, and cell culture supernatants. First, glycogen is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-570 nm/em. 590-600 nm. Glycogen levels in unknown samples are determined based on the provided glycogen standard curve.
Description: Our Glycogen Assay Kit (Colorimetric) measures glycogen in serum, plasma, urine, lysates, and cell culture supernatants. First, glycogen is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit?s Colorimetric Probe and is detected with a spectrophotometric plate reader at 540-570nm. Glycogen levels in unknown samples are determined based on the provided glycogen standard curve.
Description: Our Phosphatidylglycerol/Cardiolipin Assay Kit measures both phosphatidylglycerol and cardiolipin in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidylglycerol and cardiolipin to glycerol, which is then phosphorlyated by glycerol kinase to yield glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidylglycerol and cardiolipin levels in unknown sampled are determined based on the provided cardiolipin standard curve.
Description: Our Starch Assay Kit (Colorimetric) measures starch in food samples. First, starch is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit?s Colorimetric Probe and is detected with a spectrophotometric plate reader at 540-570nm. Starch levels in unknown samples are determined based on the provided starch standard curve.
Description: Our Starch Assay Kit (Fluorometric) measures starch in food samples. First, starch is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-570 nm/em. 590-600 nm. Starch levels in unknown samples are determined based on the provided starch standard curve.
Description: Our Homocitrulline/Citrulline Assay Kit detects total homocitrulline/citrulline in plasma, serum, urine, or cell and tissue lysates. Samples are first treated with sodium dodecyl sulfate (SDS) and proteinase K to release free homocitrulline/citrulline residues. Assay reagents are then added, which react with homocitrulline and citrulline to produce a chromophore. The absorbance is read at 540-560nm and the levels of homocitrulline/citrulline are determined based on the provided standard curve.
Description: Our DAG (Diacylglycerol) Assay Kit measures diacylglycerol in cell lysate samples by a coupled enzymatic reaction system. First, kinase is used to phosphorylate DAG in samples, yielding phosphatidic acid. Next, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. The glycerol-3-phosphate product is then oxidized by glycerol-3-phosphate oxidase, producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. DAG levels in unknown samples are determined based on the provided DAG standard curve.
Description: Cell Biolabs? Choline Assay Kits are simple assays that measure the amount of choline present in a variety of sample types in a convenient 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, acetylcholine standards and samples. Sample choline concentrations are determined by comparison with a known choline standard and measured on a fluorescence plate reader.
Description: Cell Biolabs? Choline Assay Kits are simple assays that measure the amount of choline present in a variety of sample types in a convenient 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, acetylcholine standards and samples. Sample choline concentrations are determined by comparison with a known choline standard and measured on a colorimetric plate reader.
Description: Cell Biolabs? Creatinine Assay Kit measures creatinine levels in urine. Samples are compared to a known concentration of creatinine standard within a 96-well microtiter plate format. Samples and standards are incubated for 30 minutes with a reaction reagent which changes color from yellow to bright orange upon reacting with creatinine, forming the creatinine-picrate complex. The plate is read with a standard 96-well spectrophotometric microplate reader at 490 nm. Higher OD values correlate with high creatinine concentrations. Sample creatinine concentrations are determined by comparison with the known creatinine standards.
Description: Cell Biolabs? Total Cholesterol Assay Kits measure the total cholesterol within serum, plasma, cell lysate, or tissue samples. The assays will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme.
Description: Cell Biolabs? HDL-Cholesterol Assay Kit is a simple fluorometric assay that can measure the amounts of HDL cholesterol present in plasma or serum sampleswithin a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, cholesterol standards and unknown samples. Sample cholesterol concentrations are determined by comparison with a known cholesterol standard.
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We found that the top and down-regulated genes fell into a different gene ontology categories. In a related study we [2] we show that both genes up and down-regulated to the target Brakeless directly. Our results show that the co-repressor and co-activator Brakeless activity can produce different biological responses. Adhered to the guidelines MIAME microarray data and stored in GEO with accession numbers
