BACKGROUND public sharing of scientific data have been assumed to be more important in the era of omics. Transparency is needed for confirmation and validation, and some help inspectors in extracting the maximum value from large data sets. Thus, the filing of the database and the provision of the Minimum Information About Microarray Experiment (MIAME) (3) required by most journals as a prerequisite for review or acceptance.
METHOD In this study, the rate of submission of the data and compliance MIAME reviewed for 127 articles were included based microarray microRNA (miRNA) profiling and published from July 2011 until April 2012 in the journal that published the largest number of such articles – PLoS ONE, the Journal of Biological Chemistry, Blood and Oncogenes – along with articles from other journals 9, including Clinical Chemistry, that the smaller number of articles published array-based.
RESULTS Overall, the submission of data reported in the publication for <40% of all articles, and nearly 75% of the articles were MIAME obedient. On average, the article which includes full data submission scored significantly higher on a quality metric of articles with limited or no data submission, and studies with an adequate description of the proportional method including a larger number of repeat experiments. Finally, some articles are not MIAME compliant, data reanalysis reveals less than full support for the conclusions published, in one case leading to the retraction.
CONCLUSION These findings angle hypothesis that the reluctance to share data related to the low quality studies and investigations showed that the majority of miRNA arrays underpowered and / or potentially compromised by the lack of appropriate reporting and data submission.
Data submission and quality in microarray-based microRNA profiling.
DeltaNp63 different isoforms regulate gene expression in squamous cell carcinoma: identification of Cox-2 as a new p63 targets.
P53 homologue of p63 produced six different isoforms that are important in the development of epithelial tissues and squamous cell carcinoma of the head and neck (SCCHN). In SCCHN, p63 isoform expression is very complex, with over-expression and isoform DeltaNp63 p63beta in many tumors. Until recently, little was known about the function of the different isoforms and elucidating DeltaNp63 distinctive properties of isoform DeltaNp63 will help to explain how they affect tumor biology.
With the gene expression profile SCCHN cells over-expressing isoform DeltaNp63 we identify the different effects of the three isoforms, with DeltaNp63beta become more effective in gene induction of DeltaNp63alpha and DeltaNp63gamma, while the most effective DeltaNp63gamma suppress gene expression. Thus, tumors express low levels even DeltaNp63beta or DeltaNp63gamma may have distinct clinicopathological characteristics essential for metastasis and response to therapy. Induction of cyclooxygenase-2 (Cox-2) shown by each isoform and data independently confirmed by quantitative RT-PCR and western blotting.
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit
30009-3
Biotium
1KIT
EUR 809
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Caspase-8 IETD-R110 Fluorometric HTS Assay Kit
30012-1
Biotium
1KIT
EUR 100
Description: Minimum order quantity: 1 unit of 1KIT
Caspase-8 IETD-R110 Fluorometric HTS Assay Kit
30012-2
Biotium
1KIT
EUR 358
Description: Minimum order quantity: 1 unit of 1KIT
Caspase-8 IETD-R110 Fluorometric HTS Assay Kit
30012-3
Biotium
1KIT
EUR 1627
Description: Minimum order quantity: 1 unit of 1KIT
Steady-Luc Firefly HTS Assay Kit (12 ml)
30028-1
Biotium
1KIT
EUR 139
Description: Minimum order quantity: 1 unit of 1KIT
Steady-Luc Firefly HTS Assay Kit (100 ml)
30028-2
Biotium
1KIT
EUR 610
Description: Minimum order quantity: 1 unit of 1KIT
Steady-Luc Firefly HTS Assay Kit (10x100 ml)
30028-3
Biotium
1KIT
EUR 3145
Description: Minimum order quantity: 1 unit of 1KIT
TW,HTS,6.5MM,0.4UM,S,IND,1/2
3396
CORNING
1/pk
EUR 399
Description: Permeable Support; HTS Transwell
TW,HTS,6.5MM,0.4UM,S,BK,12/12
3397
CORNING
12/pk
EUR 1955
Description: Permeable Support; HTS Transwell
TW,HTS,6.5MM,3.0UM,S,IND,1/2
3398
CORNING
1/pk
EUR 399
Description: Permeable Support; HTS Transwell
TW,HTS,6.55MM,3.0UM,S,BK,12/12
3399
CORNING
12/pk
EUR 1955
Description: Permeable Support; HTS Transwell
3D Cell Culture HTS Cell Viability Complete Assay Kit
K948-100
Biovision
EUR 343
TW,HTS,CULTURE RESERVOIR,NT,S,BK,12PL/48
3395
CORNING
12/pk
EUR 295
Description: Permeable Support; HTS Transwell
HTS TRANSWELL,TISSUE CULTURE TREATED RESERVOIR,S,BK,12/48
4395
CORNING
12/pk
EUR 321
Description: Permeable Support; HTS Transwell
Steady-Luc Firefly HTS Assay Kit (trial size)(4 mL)
30028-T
Biotium
1KIT
EUR 94
Description: Minimum order quantity: 1 unit of 1KIT
TRANSWELL,96WL,HTS,W/3.0 MEMBRANE,S,IND,1/2
3385
CORNING
1/pk
EUR 179
Description: Permeable Support; HTS Transwell
TRANSWELL,96WL,HTS,W/3.0 MEMBRANE,S,BK,4/8
3386
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4/pk
EUR 626
Description: Permeable Support; HTS Transwell
TRANSWELL,96WL, HTS,W/5.0UM MEMBRANE,S,BK,4/8
3387
CORNING
4/pk
EUR 626
Description: Permeable Support; HTS Transwell
TRANSWELL,96WL,HTS,W/5.0 MEMBRANE,S,BK,1/2
3388
CORNING
1/pk
EUR 179
Description: Permeable Support; HTS Transwell
TRANSWELL,96WL,WHITE,HTS RECEIVER,TCT,S,BK,10/10
3783
CORNING
10/pk
EUR 158
Description: Permeable Support; HTS Transwell
Human Breast Slide (Fibroadenoma) (5 slides/pk)
HTS-10004
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1 pk
EUR 286
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1 pk
EUR 286
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HTS-10010
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1 pk
EUR 286
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HTS-10012
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1 pk
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HTS-10013
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HTS-10103
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HTS-10105
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HTS-10106
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EUR 286
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HTS-10220
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1 pk
EUR 286
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HTS-10222
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1 pk
EUR 286
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HTS-10246
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1 pk
EUR 286
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HTS-10403
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1 pk
EUR 286
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HTS-10405
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1 pk
EUR 286
Human Kidney Slide (Papillary Renal Cell Carcinoma) (5 slides/pk)
HTS-10407
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1 pk
EUR 286
Human Kidney Slide (Renal Cell Carcinoma) (5 slides/pk)
HTS-10414
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1 pk
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Human Colorectal Slide (Normal) (5 slides/pk)
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1 pk
EUR 286
Human Colorectal Slide (Benign) (5 slides/pk)
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EUR 286
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HTS-10705
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HTS-10710
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HTS-10719
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1 pk
EUR 286
Human Stomach Tissue Slide (Normal) (5 slides/pk)
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1 pk
EUR 286
Human Stomach Tissue Slide (Benign) (5 slides/pk)
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1 pk
EUR 286
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1 pk
EUR 286
Human Stomach Tissue Slide (Mucinous adenocarcinoma) (5 slides/pk)
HTS-10810
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1 pk
EUR 286
Human Stomach Tissue Slide (Signet Ring Cell Adenocarcinoma) (5 slides/pk)
HTS-10811
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1 pk
EUR 286
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HTS-10814
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1 pk
EUR 286
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HTS-10826
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1 pk
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1 pk
EUR 286
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HTS-10900
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1 pk
EUR 286
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EUR 286
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HTS-10903
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1 pk
EUR 286
Human Thyroid Tissue Slide (Benign) (5 slides/pk)
HTS-11002
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1 pk
EUR 286
Human Thyroid Tissue Slide (Follicular Carcinoma) (5 slides/pk)
HTS-11006
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1 pk
EUR 286
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HTS-11007
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1 pk
EUR 286
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HTS-11018
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1 pk
EUR 286
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HTS-11101
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1 pk
EUR 286
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HTS-11105
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1 pk
EUR 286
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HTS-11106
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1 pk
EUR 286
Human Ovary Tissue Slide (Normal) (5 slides/pk)
HTS-11201
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1 pk
EUR 286
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HTS-11202
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1 pk
EUR 286
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HTS-11203
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1 pk
EUR 286
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HTS-11206
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1 pk
EUR 286
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HTS-11207
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1 pk
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HTS-11210
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1 pk
EUR 286
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HTS-11304
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1 pk
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HTS-11305
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1 pk
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HTS-11307
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1 pk
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1 pk
EUR 286
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HTS-11402
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1 pk
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Human Uterus Tissue Slide (Adenocarcinoma) (5 slides/pk)
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HTS-11407
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Human Uterus Tissue Slide (Spindle cell tumor) (5 slides/pk)
HTS-11418
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HTS-11419
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HTS-11432
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HTS-11504
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HTS-11515
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HTS-11521
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HTS-11525
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HTS-11533
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HTS-11601
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HTS-11815
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Human Small Intestine Tissue Slide (Squamous Cell Carcinoma) (5 slides/pk)
HTS-11824
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HTS-11901
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HTS-11903
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HTS-11905
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HTS-11913
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HTS-11961
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HTS-11963
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HTS-12109
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HTS-12128
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HTS-12204
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Human Soft Tissue Slide (Adipose Lipoma) (5 slides/pk)
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Human Soft Tissue Slide (Skeletal Muscle Normal) (5 slides/pk)
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No direct binding of DeltaNp63 to Cox-2 promoter could be seen, can not be evidence for Cox-2 induction as a consequence of activated NF-kappaB pathway responses found. Known as Cox-2 inhibiting response to radiotherapy in patients with SCCHN, the data shows an additional mechanism through which DeltaNp63 act to promote cell survival and affect the therapeutic response SCCHN. MIAME-compliant data has been stored in the database MIAME Express
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