BACKGROUND public sharing of scientific data have been assumed to be more important in the era of omics. Transparency is needed for confirmation and validation, and some help inspectors in extracting the maximum value from large data sets. Thus, the filing of the database and the provision of the Minimum Information About Microarray Experiment (MIAME) (3) required by most journals as a prerequisite for review or acceptance.
METHOD In this study, the rate of submission of the data and compliance MIAME reviewed for 127 articles were included based microarray microRNA (miRNA) profiling and published from July 2011 until April 2012 in the journal that published the largest number of such articles – PLoS ONE, the Journal of Biological Chemistry, Blood and Oncogenes – along with articles from other journals 9, including Clinical Chemistry, that the smaller number of articles published array-based.
RESULTS Overall, the submission of data reported in the publication for <40% of all articles, and nearly 75% of the articles were MIAME obedient. On average, the article which includes full data submission scored significantly higher on a quality metric of articles with limited or no data submission, and studies with an adequate description of the proportional method including a larger number of repeat experiments. Finally, some articles are not MIAME compliant, data reanalysis reveals less than full support for the conclusions published, in one case leading to the retraction.
CONCLUSION These findings angle hypothesis that the reluctance to share data related to the low quality studies and investigations showed that the majority of miRNA arrays underpowered and / or potentially compromised by the lack of appropriate reporting and data submission.
Data submission and quality in microarray-based microRNA profiling.
DeltaNp63 different isoforms regulate gene expression in squamous cell carcinoma: identification of Cox-2 as a new p63 targets.
P53 homologue of p63 produced six different isoforms that are important in the development of epithelial tissues and squamous cell carcinoma of the head and neck (SCCHN). In SCCHN, p63 isoform expression is very complex, with over-expression and isoform DeltaNp63 p63beta in many tumors. Until recently, little was known about the function of the different isoforms and elucidating DeltaNp63 distinctive properties of isoform DeltaNp63 will help to explain how they affect tumor biology.
With the gene expression profile SCCHN cells over-expressing isoform DeltaNp63 we identify the different effects of the three isoforms, with DeltaNp63beta become more effective in gene induction of DeltaNp63alpha and DeltaNp63gamma, while the most effective DeltaNp63gamma suppress gene expression. Thus, tumors express low levels even DeltaNp63beta or DeltaNp63gamma may have distinct clinicopathological characteristics essential for metastasis and response to therapy. Induction of cyclooxygenase-2 (Cox-2) shown by each isoform and data independently confirmed by quantitative RT-PCR and western blotting.
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1 pk
EUR 343.2
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Alpha Diagnostics
1 pk
EUR 343.2
No direct binding of DeltaNp63 to Cox-2 promoter could be seen, can not be evidence for Cox-2 induction as a consequence of activated NF-kappaB pathway responses found. Known as Cox-2 inhibiting response to radiotherapy in patients with SCCHN, the data shows an additional mechanism through which DeltaNp63 act to promote cell survival and affect the therapeutic response SCCHN. MIAME-compliant data has been stored in the database MIAME Express
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