Laboratory for Genomics and Bioinformatics

Laboratory for Genomics and Bioinformatics
	Lee H. Pratt	Marie-Michèle Cordonnier-Pratt

Recipes and Protocols
Forms		Protocols
Sug5 384-well cycle sequencing Sug3 384-well cycle sequencing JenRev 384-well cycle sequencing polyT 384-well cycle sequencing T7 384-well cycle sequencing Mini-prep form (front) Mini-prep form (back) Sequencing summary - template DNA quantification - template Primer solutions from powder		Lambda zap mass excision Electroporation Preparation of NZY-AMP agar plates Picking transfected SOLR cells DNA mini-prep 384-well cycle sequence/G-50 cleanup ABI3700 setup G-50 plate preparation Plating with glass beads Picking cells with Mantis
		Recipes
ABI3700 and Related		Recipes
ABI3700 and Related		Antibiotics Media Freezing medium Maltose - 10% MgSO4 - 100 mM SM buffer 5X reaction buffer for master mix Alkaline lysis mini-prep solutions
ABI3700 reformatting and related Dye blob examples User form for ABI3700 ABI3700 plate editor -96 samples ABI3700 plate editor - 384 samples
Miscellaneous
Bacterial growth curves pME18S vector/primer sequences pBluescript SK(-) vector/primer sequences pCR2.1-TOPO vector/primer sequences