Laboratory for Genomics and Bioinformatics

 
 

Lee H. Pratt

Marie-Michèle Cordonnier-Pratt
 
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Floral-Induced Meristems (FM1)

Mature S. propinquum plants were transferred from a greenhouse to a growth chamber for 15 days. During these 15 days, plants received 16 hr darkness and 8 hr light each day (flowering is induced by short-day conditions). Plants were returned to the greenhouse under natural long days during late April/early May. After 16 days, meristems were harvested and used as the source of RNA for the cDNA library.

 
 

QPSE16 LenNVE >=100 gives the number of sequences that met our criteria for submission to GenBank. Because more than one sequence was occasionally obtained from a cDNA, the number of non-replicate sequences is also provided.

The average Phred Q16 and Q20 lengths are contiguous; if sequence quality declined, but then improved again, the additional high-quality base calls are not included in these numbers.

The number and proportion unique derives from clustering of all 3' sequences from this one library.