Lee H. Pratt

Sequences are retrieved dynamically from an Oracle 9i database, which is updated nightly. Thus, sequences are available within 24 hours after the raw data (trace files) have been processed.

Sequences may be accessed in two different ways:

1) Select the link at the top of the page (Specify a sequence . . .) and then enter either the GenBank Accession Number or our own Sequence Name.

2) Select the library from which you want to view a sequence. Libraries are accompanied by a brief description of the RNA source. If additional library information is desired, go to the Library page.
     After selecting the desired library, the following page displays available 96-well plates from which sequences were obtained. Go to the Sequence Name page to learn how to identify the block you might want.
     Select a block, after which you will have a list of all sequences, both 5' and 3', as well as GenBank accession numbers and locations on the rice genome at Gramene. Both of the latter are hyperlinks, going either to the GenBank accession or to the appropriate location in the rice genome at Gramene.
     For those sequences included in the Milestone Unigene Set, there will also be links to those contigs in the column labeled "3' Cluster".
     If you click on a sequence to view it, you will see it in text format, followed by information describing the color coding.
     If you click on a 3' cluster, you will see the consensus sequence followed by individual members of the cluster. GenBank and Gramene links are also included. The "Anchor Sequence" defines the cDNA clone that serves as the type clone for that cluster.